Effect of Incorporating Nano-Pearl and Nano-Seashell Particles into Fluoride-based Pits and Fissure Sealant on Enamel Remineralization of Permanent Extracted Molars: An In Vitro Study.

Objective: This in-vitro study evaluated the remineralizing effect of nano-pearl and nano-seashell incorporated into fluoride-based pits-and-fissures sealants by assessing enamel surface microhardness elemental analysis. Materials Methods: 75 extracted third molars were randomly allocated to one following 5 groups fluoride containing sealant: 10% nano-pearl; 15% nano-seashell; or plain sealant. After 2 weeks dynamic pH-cycling using Pepsi as demineralizing solution; 10 each group subjected assessment, while Energy Dispersive X-ray Analysis. Results: Teeth treated with reported highest percentage increase (20.5%) teeth sealant showed a decrease (-11.62 %). The XRD pattern results confirmed presence aragonite calcite polymorphs CaCO3 in powder powder. Calcium ions recorded significantly higher value pits fissure Conclusion: incorporation % 15 particles improved well mineral content.